Journal: Vaccines

Article Title: Evaluating the Compatibility of Three Aluminum Salt-Adjuvanted Recombinant Protein Antigens (Trivalent NRRV) Combined with a Mock Trivalent Sabin-IPV Vaccine: Analytical and Formulation Challenges

doi: 10.3390/vaccines12101102

Figure Lengend Snippet: Stability profiles of AH-adsorbed t-sIPV antigens (types 1, 2, and 3) in the presence and absence of different antimicrobial preservatives (APs) as measured by Sabin D-antigen competitive ELISAs. The relative antigen–antibody binding of each AH-adsorbed sIPV antigen to an antigen-specific antibody is shown as a percentage concentration relative to time zero values after storage at 2–8 °C and 15 °C for 3 months, and 37 °C for 1 day, for sIPV1 (Panels ( A , D , G )), sIPV2 (Panels ( B , E , H )), and sIPV3 (Panels ( C , F , I )) in the presence of the indicated AP. TH—thimerosal, 2-PE—2-phenoxy ethanol, PH—phenol, CB—chlorobutanol, MC—m-cresol, BA—benzyl alcohol, MP—methyl paraben, and PP—propyl paraben. Data are presented as the mean ± SD (n = 4).

Article Snippet: Capture and detection antibodies used in the competition enzyme-linked immunoassays (ELISAs), system suitability controls, and reference standards were purchased from Batavia Biosciences (The Netherlands) for three sIPV antigens and Precision Antibody (Columbia, MD, USA) for the three NRRV antigens.

Techniques: Binding Assay, Concentration Assay

Journal: Frontiers in Immunology

Article Title: The Pathogenicity of BPI-ANCA in a Patient With Systemic Vasculitis

doi: 10.3389/fimmu.2019.01334

Figure Lengend Snippet: Laboratory data.

Article Snippet: Further ELISA assay (ANCA panel kit, Euro Diagnostica) revealed that the antigen for C-ANCA was BPI (titer; 6.5 O.D. ratio, ) and other atypical ANCAs including azurocidin, cathepsin G, elastase, lactoferrin, and lysozyme were negative.

Techniques:

Journal: Frontiers in Immunology

Article Title: The Pathogenicity of BPI-ANCA in a Patient With Systemic Vasculitis

doi: 10.3389/fimmu.2019.01334

Figure Lengend Snippet: BPI-ANCAs induce NETs with hyper citrullinated histones. (A) Representative NETs staining of healthy or BPI-AAV patient IgGs-stimulated neutrophils using CitH3 (red) and DAPI (BLUE). (B) Representative Sytox-positive neutrophils live image. From the left, healthy IgGs ( n = 3), MPO-AAV patient IgGs ( n = 5), BPI-AAV patient IgGs. Right figure shows Sytox-positive areas quantified by Image J. (C) Representative CitH3-positive NETs and Sytox-positive neutrophils treated with TNFα, TNFα+mBPI-Ab, TNFα+mBPI-Ab+rBPI. Right figures show CitH3-positive area (upper) and Sytox-positive area (lower) under each situation. Data represent the mean±SEM of three independent experiments and were analyzed using unpaired t -test (PRISM software, GraphPad). * p < 0.05. (D) BPI expression with or without TNFα stimulation. Green; BPI, BLUE; DAPI. (E) Sytox positivity of neutrophils treated with 3% serum, or 3% IgG depleted serum of patients with BPI-AAV, MPO-AAV ( n = 3) and healthy control ( n = 3). Scale bar in all figures, 50 mm. CitH3, citrullinated histone3; DAPI, 49,6-diamidin-2-phenylindol; BPI, bactericidal/permeability increasing protein; ANCA, anti neutrophil cytoplasmic antibody; AAV, ANCA associated vasculitis; IgG, immunoglobulin G; TNFα, tumor necrosis factor alpha; mBPI-Ab, monoclonal BPI antibody; rBPI, recombinant BPI.

Article Snippet: Further ELISA assay (ANCA panel kit, Euro Diagnostica) revealed that the antigen for C-ANCA was BPI (titer; 6.5 O.D. ratio, ) and other atypical ANCAs including azurocidin, cathepsin G, elastase, lactoferrin, and lysozyme were negative.

Techniques: Staining, Software, Expressing, Control, Permeability, Recombinant

Journal: PLoS ONE

Article Title: Investigation of the performance of serological assays used for Lyme disease testing in Australia

doi: 10.1371/journal.pone.0214402

Figure Lengend Snippet: Number and B . burgdorferi IgG antibody status of specimens provided by participating laboratories.

Article Snippet: Trinity Biotech B . burgdorferi ELISA (IgM/IgG) Test System (Trinity Biotech ELISA) , Native , MarDX Diagnostics Inc California, United States .

Techniques:

Journal: PLoS ONE

Article Title: Investigation of the performance of serological assays used for Lyme disease testing in Australia

doi: 10.1371/journal.pone.0214402

Figure Lengend Snippet: Name, manufacturer and preparation of antigens used in the assays included in the study.

Article Snippet: Trinity Biotech B . burgdorferi ELISA (IgM/IgG) Test System (Trinity Biotech ELISA) , Native , MarDX Diagnostics Inc California, United States .

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Western Blot

Journal: PLoS ONE

Article Title: Investigation of the performance of serological assays used for Lyme disease testing in Australia

doi: 10.1371/journal.pone.0214402

Figure Lengend Snippet: The sensitivity, specificity, 95% confidence intervals (CI) and delta values of assays in known B . burgdorferi IgG positive and negative specimen panels respectively. Equivocal results are considered negative for sensitivity and positive for specificity estimations.

Article Snippet: Trinity Biotech B . burgdorferi ELISA (IgM/IgG) Test System (Trinity Biotech ELISA) , Native , MarDX Diagnostics Inc California, United States .

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Investigation of the performance of serological assays used for Lyme disease testing in Australia

doi: 10.1371/journal.pone.0214402

Figure Lengend Snippet: The positive and negative agreement of the study assays in specimens with presumed B . burgdorferi positive and negative IgG status respectively. Equivocal results obtained in the study are considered negative for estimation of positive agreement and positive for estimation of negative agreement.

Article Snippet: Trinity Biotech B . burgdorferi ELISA (IgM/IgG) Test System (Trinity Biotech ELISA) , Native , MarDX Diagnostics Inc California, United States .

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Investigation of the performance of serological assays used for Lyme disease testing in Australia

doi: 10.1371/journal.pone.0214402

Figure Lengend Snippet: Agreement between specimens’ results obtained in the clinical laboratory and results obtained in this study in the same assay.

Article Snippet: Trinity Biotech B . burgdorferi ELISA (IgM/IgG) Test System (Trinity Biotech ELISA) , Native , MarDX Diagnostics Inc California, United States .

Techniques: Enzyme-linked Immunosorbent Assay